Induki Mutasi dengan Sinar UV-C dan Seleksi In Vitro Kalus Kedelai yang Toleran Kekeringan
DOI:
https://doi.org/10.25077/jagur.7.2.121-126.2025Keywords:
in vitro, soybean, drought, mutation, ultraviolet light CAbstract
Soybean (Glycine max. L) is a strategic commodity that plays a major role in providing nutritious food. One effort to increase soybean production is through the use of superior varieties. Dega-1 is a soybean variety that is very sensitive to drought so that it is necessary to develop soybeans cultivated in dry land which can be attempted through mutation breeding. The purpose of this study was to determine the duration of ultraviolet C light irradiation that can produce mutants and to obtain putative drought-tolerant mutants through ultraviolet C light mutation induction and in vitro selection (PEG 25%). This study was conducted from April to July 2024 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University. This study was arranged in a Randomized Block Design with treatment duration of UV C light irradiation λ 254 nm with 10 treatment levels including no radiation, 15 minutes, 30 minutes, 45 minutes, 60 minutes, 75 minutes, 90 minutes, 105 minutes, 120 minutes, and 135 minutes. Each treatment consisted of 5 groups. The observation data were analyzed using simple statistical analysis using the mean and standard deviation calculations. The results showed that the highest percentage of putative drought-tolerant soybean mutants (PEG 6000 25%) was produced by 45 minutes of UV C irradiation, namely 3.33%. These mutants emerged from blackish-brown callus and developed into yellow callus and also regenerated into somatic embryos.
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References
Badan Pangan Nasional. (2022). Laporan Kinerja Badan Pangan Nasional Tahun Anggaran 2022. Badan Pangan Nasional.
Budi, R. S., Suliansyah, I., Yusniwati, & Sobrizal. (2019). Perbaikan Genetik Padi Gogo Beras Merah Sumatera Utara melalui Pemuliaan Mutasi. Jurnal Ilmiah Aplikasi Isotop dan Radiasi, 15(1).
Deng, C., Zhang, Y., Wang, B., Wang, H., Xue, P., Cao, Y., Sun, L., Cheng, S., Cao, L., & Chen, D. (2023).
Identification and Fine Mapping of Osdsm3, a Drought-Sensitive Gene in Rice (Oryza sativa L.). Agronomy, 13(9), 2241. https://doi.org/10.3390/agronomy13092241
Haryanti, S. (2024). Iradiasi Sinar Ultraviolet-C Untuk Peningkatan Kelenjer Minyak dan Ketahanan Penakit Layu Bakteri pada Nilam (Pogostemon cablin Benth.) [Thesis]. Universitas Andalas.
International Atomic Energy Agency, (IAEA). (2023). Mutant Variety Database [Dataset]. https://nucleus.iaea.org/sites/mvd/SitePages/Home.aspx
Lestari, E. G. (2021). Aplikasi Induksi Mutasi Untuk Pemuliaan Tanaman Hias. Jurnal ilmu-ilmu Hayati, 20(1), 1–12. https://doi.org/10.14203/beritabiologi.v20i1.3991
Mansoor, S., Ali Wani, O., Lone, J. K., Manhas, S., Kour, N., Alam, P., Ahmad, A., & Ahmad, P. (2022). Reactive Oxygen Species in Plants: From Source to Sink. Antioxidants, 11(2), 225. https://doi.org/10.3390/antiox11020225
Mendez-Hernandez, H. A., Ledezma-Rodriguez, M., Avilez-Montalvo, R. N., Juarez-Gomez, Y. L., Skeete, A., Avilez-Montalvo, J., De-la-Peña, C., & Loyola-Vargas, V. M. (2019). Signaling Overview of Plant Somatic Embryogenesis. Frontiers in Plant Science, 10, 77. https://doi.org/10.3389/fpls.2019.00077
Nastiti, D. H. (2022). Induksi Embrio Somatik Sekunder Kedelai (Glycine max (L.) Merrill) Varietas Dega dengan Pemberian Kombinasi 2,4-D dan NAA secara In Vitro. Universitas Andalas.
Nichols, S. N., Hofmann, R. W., & Williams, W. M. (2015). Physiological drought resistance and accumulation of leaf phenolics in white clover interspecific hybrids. Environmental and Experimental Botany, 119, 40–47. https://doi.org/10.1016/j.envexpbot.2015.05.014
Ouyang, W., Chen, L., Ma, J., Liu, X., Chen, H., Yang, H., Guo, W., Shan, Z., Yang, Z., Chen, S., Zhan, Y., Zhang, H., Cao, D., & Zhou, X. (2022). Identification of Quantitative Trait Locus and Candidate Genes for Drought Tolerance in a Soybean Recombinant Inbred Line Population. International Journal of Molecular Sciences, 23(18), 10828. https://doi.org/10.3390/ijms231810828
Pamungkas, S. S. T., Suwarto, Suprayogi, & Farid, N. (2022). Drought Stress: Responses and Mechanism in Plants. Reviews in Agricultural Science, 10(0), 168–185. https://doi.org/10.7831/ras.10.0_168
Pujiwati, H., Romeida, A., Widodo, Prameswari, W., Husna, M. L., & Anandyawati. (2021). Rapid Screening Tolerance of 19 Soybean Varieties to Drought in the Germination Phase: International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020), Bengkulu, Sumatra, Indonesia, Indonesia. https://doi.org/10.2991/absr.k.210609.044
Ritung, S., Husen, E., Agus, F., & Nursyamsi, D. (with IAARD Press). (2015). Sumber daya lahan pertanian Indonesia: Luas, penyebaran, dan potensi ketersediaan. Indonesian Agency for Agricultural Research and Development Press.
Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., & Zheng, B. (2019). Response of Phenylpropanoid Pathway and the Role of Polyphenols in Plants under Abiotic Stress. Molecules, 24(13), 2452. https://doi.org/10.3390/molecules24132452
Sulastri, Rosdayanti, H., & Nawfetrias, W. (2021). Potensi Pembentukan Alfalfa (Medicago sativa) Toleran Kering Melalui Induksi Mutasi Iradiasi Sinar UV-C dan Seleksi Variasi Somaklonal. Prosiding Seminar Nasional Komisi Nasional Sumber Daya Genetik, 693–705.
Surmaini, E., & Faqih, A. (2016). Kejadian Iklim Ekstrem dan Dampaknya Terhadap Pertanian Tanaman Pangan di Indonesia. Jurnal Sumberdaya Lahan, 10(2), 115–128.
Ubudiyah, I. W. A., & Nurhidayati, T. (2013). Respon Kalus Beberapa Varietas Padi (Oryza sativa L.) pada Kondisi Cekaman Salinitas (NaCl) secara In Vitro. Jurnal Sains Dan Seni Pomits, 2(2), 2337–3520.
Yang, C., Huang, Y., Lv, P., Antwi-Boasiako, A., Begum, N., Zhao, T., & Zhao, J. (2022). NAC Transcription Factor GmNAC12 Improved Drought Stress Tolerance in Soybean. International Journal of Molecular Sciences, 23(19), 12029. https://doi.org/10.3390/ijms231912029
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